Table 2.
Calculated EC50 values of each SRC peptide for the ESR1 in response to each ligand in the TR-FRET assays. Dose–response curves were generated by plotting emission ratios (y axis) against ligand concentration (x axis) using SigmaPlot 11 (Systat Software, Inc., San Jose, CA). Curves were analyzed by transforming the x-axis to a logarithmic scale and applying a nonlinear regression curve using a sigmoidal-dose response with variable slope and EC50 values calculated using SigmaPlot 11.
| SRC-1 (nM) |
SRC-3 (nM) |
||||||
|---|---|---|---|---|---|---|---|
| Ligand | (1) | (2) | (3) | (4) | (1) | (2) | (3) |
| 17β-Estradiol | 8.44 | 5.08 | 2.61 | – | – | 7.19 | 2.74 |
| 4-OH-Tamoxifen | – | – | – | – | – | – | – |
| Genistein | – | 25.0 | – | 511 | 17.0 | 38.8 | 72.4 |
| Bisphenol-A | – | 32.3 | 3230 | – | – | – | – |