Table 3.
Proteins identified from ESR1 transcriptional complexes of MCF-7 cells after exposure to 10 nM 17β-estradiol (E2), 1 μM bisphenol-A (BPA), or 1 μM genistein (GEN) for 3.5 h, excluding proteins identified in control group. Proteins are organized by treatment and number of identifications among triplicate samples within a treatment group (IDs).
| Ligand | UniProt Accession Number | Gene Symbol | Protein Name | Molecular Weight | IDs |
|---|---|---|---|---|---|
| E2 | P03372 | ESR1 | Estrogen receptor | 66 kDa | 3 |
| Q9UBS4 | DNAJB11 | DnaJ homolog subfamily B member 11‡ | 41 kDa | 3 | |
| P62330 | ARF6 | ADP-ribosylation factor 6‡ | 20 kDa | 2 | |
| Q13185 | CBX3 | Chromobox protein homolog 3‡ | 21 kDa | 2 | |
| P35222 | CTNNB1 | Catenin beta-1† | 85 kDa | 2 | |
| P08574 | CYC1 | Cytochrome c1, heme protein, mitochondrial‡ | 35 kDa | 2 | |
| Q14315 | FLNC | Filamin-C‡ | 291 kDa | 2 | |
| P50148 | GNAQ | Guanine nucleotide-binding protein G(q) subunit alpha† | 42 kDa | 2 | |
| O15049 | N4BP3 | NEDD4-binding protein 3 | 60 kDa | 2 | |
| O75489 | NDUFS3 | NADH dehydrogenase [ubiquinone] iron–sulfur protein 3, mitochondrial‡ | 30 kDa | 2 | |
| P61289 | PSME3 | Proteasome activator complex subunit 3† | 30 kDa | 2 | |
| Q9Y2W1 | THRAP3 | Thyroid hormone receptor-associated protein 3‡ | 109 kDa | 2 | |
| P07311 | ACYP1 | Acylphosphatase-1 | 11 kDa | 1 | |
| P20648 | ATP4A | Potassium-transporting ATPase alpha chain 1‡ | 114 kDa | 1 | |
| Q5SY80 | C1orf101 | Uncharacterized protein C1orf101 | 110 kDa | 1 | |
| Q4AC94 | C2CD3 | C2 domain-containing protein 3 | 260 kDa | 1 | |
| Q5M9N0 | CCDC158 | Coiled-coil domain-containing protein 158 | 127 kDa | 1 | |
| Q96A33 | CCDC47 | Coiled-coil domain-containing protein 47 | 56 kDa | 1 | |
| P19256 | CD58 | Lymphocyte function-associated antigen 3 | 28 kDa | 1 | |
| Q9UNH5 | CDC14A | Dual specificity protein phosphatase CDC14A‡ | 67 kDa | 1 | |
| Q96JB2 | COG3 | Conserved oligomeric Golgi complex subunit 3 | 94 kDa | 1 | |
| Q96EY1 | DNAJA3 | DnaJ homolog subfamily A member 3, mitochondrial‡ | 52 kDa | 1 | |
| O75923 | DYSF | Dysferlin | 237 kDa | 1 | |
| Q96C19 | EFHD2 | EF-hand domain-containing protein D2 | 27 kDa | 1 | |
| Q14156 | EFR3A | Protein EFR3 homolog A | 93 kDa | 1 | |
| P05198 | EIF2S1 | Eukaryotic translation initiation factor 2 subunit 1‡ | 36 kDa | 1 | |
| Q8N8S7 | ENAH | Protein enabled homolog‡ | 67 kDa | 1 | |
| O14908 | GIPC1 | PDZ domain-containing protein GIPC1 | 36 kDa | 1 | |
| P00367 | GLUD1 | Glutamate dehydrogenase 1, mitochondrial‡ | 61 kDa | 1 | |
| Q03113 | GNA12 | Guanine nucleotide-binding protein subunit alpha-12‡ | 44 kDa | 1 | |
| P16520 | GNB3 | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3‡ | 37 kDa | 1 | |
| O75487 | GPC4 | Glypican-4‡ | 62 kDa | 1 | |
| P16402 | HIST1H1D | Histone H1.3‡ | 22 kDa | 1 | |
| Q9H910 | HN1L | Hematological and neurological expressed 1-like protein | 20 kDa | 1 | |
| Q99714 | HSD17B10 | 3-hydroxyacyl-CoA dehydrogenase type-2 | 27 kDa | 1 | |
| P48741 | HSPA7 | Putative heat shock 70 kDa protein 7‡ | 40 kDa | 1 | |
| P50213 | IDH3A | Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial‡ | 40 kDa | 1 | |
| Q27J81 | INF2 | Inverted formin-2 | 136 kDa | 1 | |
| Q15046 | KARS | Lysine–tRNA ligase‡ | 68 kDa | 1 | |
| Q5VWX1 | KHDRBS2 | KH domain-containing, RNA-binding, signal transduction-associated protein 2‡ | 39 kDa | 1 | |
| Q7Z3Y7 | KRT28 | Keratin, type I cytoskeletal 28 | 51 kDa | 1 | |
| Q14525 | KRT33B | Keratin, type I cuticular Ha3-II | 46 kDa | 1 | |
| Q71F78 | LCA10 | Putative lung carcinoma-associated protein 10 | 17 kDa | 1 | |
| Q03252 | LMNB2 | Lamin-B2‡ | 68 kDa | 1 | |
| Q32MZ4 | LRRFIP1 | Leucine-rich repeat flightless-interacting protein 1‡ | 89 kDa | 1 | |
| P61326 | MAGOH | Protein mago nashi homolog‡ | 17 kDa | 1 | |
| O60524 | NEMF | Nuclear export mediator factor NEMF‡ | 123 kDa | 1 | |
| Q9BSD7 | NTPCR | Cancer-related nucleoside-triphosphatase | 21 kDa | 1 | |
| Q99571 | P2RX4 | P2X purinoceptor 4 | 43 kDa | 1 | |
| Q86U42 | PABPN1 | Polyadenylate-binding protein 2‡ | 33 kDa | 1 | |
| O43252 | PAPSS1 | Bifunctional 3′-phosphoadenosine 5′-phosphosulfate synthase 1 | 71 kDa | 1 | |
| Q15120 | PDK3 | [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3, mitochondrial | 47 kDa | 1 | |
| Q8N0Y7 | PGAM4 | Probable phosphoglycerate mutase 4 | 29 kDa | 1 | |
| A1L390 | PLEKHG3 | Pleckstrin homology domain-containing family G member 3‡ | 134 kDa | 1 | |
| Q9UHX1 | PUF60 | Poly(U)-binding-splicing factor PUF60‡ | 60 kDa | 1 | |
| Q15276 | RABEP1 | Rab GTPase-binding effector protein 1 | 99 kDa | 1 | |
| Q16576 | RBBP7 | Histone-binding protein RBBP7† | 48 kDa | 1 | |
| Q8WZ73 | RFFL | E3 ubiquitin-protein ligase rififylin | 41 kDa | 1 | |
| P04279 | SEMG1 | Semenogelin-1 | 52 kDa | 1 | |
| Q15637 | SF1 | Splicing factor 1† | 68 kDa | 1 | |
| P53007 | SLC25A1 | Tricarboxylate transport protein, mitochondrial | 34 kDa | 1 | |
| Q9NTJ3 | SMC4 | Structural maintenance of chromosomes protein 4‡ | 147 kDa | 1 | |
| Q92485 | SMPDL3B | Acid sphingomyelinase-like phosphodiesterase 3b | 51 kDa | 1 | |
| Q8NB12 | SMYD1 | SET and MYND domain-containing protein 1 | 57 kDa | 1 | |
| P62306 | SNRPF | Small nuclear ribonucleoprotein F‡ | 10 kDa | 1 | |
| Q9BX66 | SORBS1 | Sorbin and SH3 domain-containing protein 1‡ | 143 kDa | 1 | |
| Q07617 | SPAG1 | Sperm-associated antigen 1 | 104 kDa | 1 | |
| P11277 | SPTB | Spectrin beta chain, erythrocytic‡ | 246 kDa | 1 | |
| Q9Y5M8 | SRPRB | Signal recognition particle receptor subunit beta‡ | 30 kDa | 1 | |
| Q8WUY1 | THEM6 | Protein THEM6 | 24 kDa | 1 | |
| Q6UW68 | TMEM205 | Transmembrane protein 205 | 21 kDa | 1 | |
| O94826 | TOMM70A | Mitochondrial import receptor subunit TOM70 | 67 kDa | 1 | |
| Q9C040 | TRIM2 | Tripartite motif-containing protein 2 | 82 kDa | 1 | |
| P43897 | TSFM | Elongation factor Ts, mitochondrial‡ | 35 kDa | 1 | |
| Q13885 | TUBB2A | Tubulin beta-2 A chain† | 50 kDa | 1 | |
| P08670 | VIM | Vimentin‡ | 54 kDa | 1 | |
| BPA | P03372 | ESR1 | Estrogen receptor | 66 kDa | 3 |
| P29279 | CTGF | Connective tissue growth factor† | 38 kDa | 1 | |
| P49448 | GLUD2 | Glutamate dehydrogenase 2, mitochondrial | 61 kDa | 1 | |
| Q03113 | GNA12 | Guanine nucleotide-binding protein subunit alpha-12‡ | 44 kDa | 1 | |
| Q58FG0 | HSP90AA5P | Putative heat shock protein HSP 90-alpha A5 | 39 kDa | 1 | |
| Q86UX2 | ITIH5 | Inter-alpha-trypsin inhibitor heavy chain H5 | 105 kDa | 1 | |
| Q9BW19 | KIFC1 | Kinesin-like protein KIFC1 | 74 kDa | 1 | |
| Q14CN4 | KRT72 | Keratin, type II cytoskeletal 72 | 56 kDa | 1 | |
| P78386 | KRT85 | Keratin, type II cuticular Hb5 | 56 kDa | 1 | |
| O43813 | LANCL1 | LanC-like protein 1 | 45 kDa | 1 | |
| Q13203 | MYBPH | Myosin-binding protein H | 52 kDa | 1 | |
| P0CAP1 | MYZAP | Myocardial zonula adherens protein | 54 kDa | 1 | |
| P54920 | NAPA | Alpha-soluble NSF attachment protein‡ | 33 kDa | 1 | |
| P15531 | NME1 | Nucleoside diphosphate kinase A† | 17 kDa | 1 | |
| Q04721 | NOTCH2 | Neurogenic locus notch homolog protein 2 | 265 kDa | 1 | |
| Q7Z3S9 | NOTCH2NL | Notch homolog 2 N-terminal-like protein | 26 kDa | 1 | |
| Q99497 | PARK7 | Protein DJ-1 | 20 kDa | 1 | |
| P16435 | POR | NADPH–cytochrome P450 reductase | 77 kDa | 1 | |
| P36873 | PPP1CC | Serine/threonine-protein phosphatase PP1-gamma catalytic subunit | 37 kDa | 1 | |
| Q6WKZ4 | RAB11FIP1 | Rab11 family-interacting protein 1 | 137 kDa | 1 | |
| Q96DA2 | RAB39B | Ras-related protein Rab-39B | 25 kDa | 1 | |
| Q9H930 | SP140L | Nuclear body protein SP140-like protein | 67 kDa | 1 | |
| Q6UW68 | TMEM205 | Transmembrane protein 205 | 21 kDa | 1 | |
| Q9NZR1 | TMOD2 | Tropomodulin-2 | 40 kDa | 1 | |
| Q9NY65 | TUBA8 | Tubulin alpha-8 chain† | 50 kDa | 1 | |
| GEN | P03372 | ESR1 | Estrogen receptor | 66 kDa | 3 |
| P55957 | BID | BH3-interacting domain death agonist | 22 kDa | 1 | |
| P16402 | HIST1H1D | Histone H1.3 | 22 kDa | 1 | |
| Q58FF8 | HSP90AB2P | Putative heat shock protein HSP 90-beta 2† | 44 kDa | 1 | |
| Q5VWX1 | KHDRBS2 | KH domain-containing, RNA-binding, signal transduction-associated protein 2 | 39 kDa | 1 | |
| Q8N1N4 | KRT78 | Keratin, type II cytoskeletal 78‡ | 57 kDa | 1 | |
| P18615 | NELFE | Negative elongation factor E | 43 kDa | 1 | |
| P60866 | RPS20 | 40S ribosomal protein S20 | 13 kDa | 1 | |
| Q6UW68 | TMEM205 | Transmembrane protein 205 | 21 kDa | 1 | |
| Q86VY4 | TSPYL5 | Testis-specific Y-encoded-like protein 5 | 45 kDa | 1 |
†
after the protein name denotes a primary relationship with ESR1
‡
denotes a secondary relationship as determined by STRING analysis.