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. Author manuscript; available in PMC: 2017 Aug 15.
Published in final edited form as: Clin Cancer Res. 2016 Apr 13;22(16):4249–4258. doi: 10.1158/1078-0432.CCR-15-2518

Figure 2. Lymphocytes are present within the tumor microenvironment.

Figure 2

(A) Representative images of patient tissue sections from confirmed cases of ovarian cancer were stained for CD3 and CD8. (B) Flow cytometry was performed to assess T cell subpopulations within the ascites. CD1d-expressing cells were treated with cell-free ascites for 4 hours, then washed extensively and cocultured with a panel of NKT cell hybridomas. Samples that caused <10% inhibition of IL-2 production by NKT cells were deemed non-inhibitory, the others are referred to as inhibitory.