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. Author manuscript; available in PMC: 2016 Aug 16.
Published in final edited form as: Biochemistry. 2016 Aug 1;55(32):4519–4532. doi: 10.1021/acs.biochem.5b01132

Figure 1. DJ-1 protein content is regulated by Grx1 in vivo.

Figure 1

(A) Western blot analyses of midbrain homogenates from WT and Grx1−/− mice probed with anti-Grx1, anti-DJ-1, and anti-actin antibodies, respectively. (B) Quantification of DJ-1 protein content (normalized to actin) in the lysates of midbrain samples from WT and Grx1−/− mice; n = 6 independent WT and Grx1−/− mice/*p<0.05. Statistical analysis was completed using Student’s T-test. Error bars represent SEM. (C) Positive correlation between the protein contents of DJ-1 and Grx1 (normalized to actin) in human PBMCs; n = 14 independent biological samples/p<0.01, R2=0.54. Statistical analysis was completed using a one-way linear regression test. (D) Positive correlation between the contents of the DJ-1 and Grx1 proteins (normalized to actin) in lysates of human post mortem midbrain tissue; n = 14 independent biological samples/p<0.01, R2=0.63. Statistical analysis was completed using a one-way linear regression test.