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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Gut. 2016 Feb 16;66(7):1197–1207. doi: 10.1136/gutjnl-2015-310341

Figure 3. Autophagy suppresses TNF-α-mediated basal cell proliferation in oesophageal organoids.

Figure 3

Murine oesophageal 3D organoids were grown ex vivo and treated from days 4–11 with 40 ng/ml TNF-α and/or 1 µg/ml CQ as indicated and subjected to H&E staining or IHC for p63 (basal cells) and cleaved LC3 (autophagy). (A) Representative images. Scale bars, 25 µm. (B) Quantification of basal cell (BC) score based upon morphology. *, p<0.05; vs. TNF-α (−) CQ (−); #, p<0.05; vs. TNF-α (+) CQ (−) and TNF-α (−) CQ (+).