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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Gut. 2016 Feb 16;66(7):1197–1207. doi: 10.1136/gutjnl-2015-310341

Figure 5. EoE-relevant Th2 cytokines promote autophagic flux in oesophageal keratinocytes.

Figure 5

EPC2-hTERT cells were stimulated with 10 ng/ml IL-4, IL-5 and IL-13 in the presence or absence of 1 µg/ml CQ as indicated. (A) After 24 hours, immunoblot determined LC3-II and p62 (densitometry shown at bottom) with β-actin as a loading control. (B) After 72 hours, live cell imaging determined GFP-LC3 puncta with representative cell images (Left, Scale bar, 20 µm) and average GFP-LC3 puncta/cell (Right). *, p<0.05 vs. IL-13 (−) CQ (−); #, p<0.05 vs. IL-13 (+) CQ (−) and IL-13 (−) CQ (+); n=3.