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. 2016 Aug 15;214(4):459–474. doi: 10.1083/jcb.201601014

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© 2016 McLaughlin et al.

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Figure 5. — Toll-6, dSARM, and FoxO are required for rapid presynaptic structural plasticity. (A–E) Representative confocal projections of NMJ6/7 in A2-3 labeled with HRP and Dlg after high K⁺ stimulation. Arrows denote nascent boutons. Bar, 10 µm. (F) Quantification of nascent boutons after high K⁺ and control stimulation paradigms. Wild type_2mM: 3.3 ± 0.2 (n = 27); wild type_90mM: 7.6 ± 0.6 (n = 31); foxO^Δ94_2mM: 3.6 ± 0.3 (n = 14); foxO^Δ94_90mM: 4.5 ± 0.4 (n = 26); Toll-6^EX13_2mM: 3.4 ± 0.4 (n = 16); Toll-6^EX13_90mM: 4.2 ± 0.4 (n = 31); Toll-6^EX13, foxO^Δ94_2mM: 3.2 ± 0.5 (n = 10); Toll-6^EX13, foxO^Δ94_90mM: 3.2 ± 0.5 (n = 11); dSARM⁴⁶²¹_2mM: 2.9 ± 0.6 (n = 7); and dSARM⁴⁶²¹_90mM: 2.9 ± 0.5 (n = 16). Wild type is OregonR. (G) Quantification of nascent boutons after high K⁺ and control stimulation paradigms. Control_2mM: 2.4 ± 0.3 (n = 17); control_90mM: 7.1 ± 0.8 (n = 18); D42>dcr-2; foxO RNAi_2mM: 2.9 ± 0.5 (n = 17); D42>dcr-2; foxO RNAi_90mM: 4.2 ± 1.1 (n = 17); D42>dcr-2; Toll-6 RNAi^#1_2mM: 3.2 ± 0.4 (n = 22); D42>dcr-2; Toll-6 RNAi^#1_90mM: 3.3 ± 0.5 (n = 18); D42>dcr-2; Toll-6 RNAi^#2_2mM: 3.0 ± 0.5 (n = 15); D42>dcr-2; Toll-6 RNAi^#2_90mM: 2.7 ± 0.4 (n = 20); D42>dcr-2; dSARM RNAi^#1_2mM: 3.7 ± 0.6 (n = 25); D42>dcr-2; dSARM RNAi^#1_90mM: 4.6 ± 0.7 (n = 29); D42>dcr-2; dSARM RNAi^#2_2mM: 3.7 ± 0.7 (n = 17); and D42>dcr-2; dSARM RNAi^#2_90mM: 5.4 ± 1.6 (n = 14). Control is D42>dcr-2. (H) Quantification of nascent boutons after high K⁺ and control stimulation paradigms. Control_2mM: 2.9 ± 0.3 (n = 27); control_90mM: 6.9 ± 0.8 (n = 29); OK6>Pav^#1_2mM: 2.5 ± 0.5 (n = 11); OK6>Pav^#1_90mM: 3.2 ± 0.6 (n = 20); OK6>Pav^#2_2mM: 3.5 ± 0.4 (n = 17); and OK6>Pav^#2_90mM: 3.3 ± 0.6 (n = 12). Control is OK6Gal4/+. Error bars are mean ± SEM. n.s., not significantly different. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Statistical comparisons above 90 mM bars are to 90 mM measurements of wild type or control.