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. 2016 Aug 15;214(4):445–458. doi: 10.1083/jcb.201604061

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© 2016 Zhou et al.

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Figure 2. — Prenylated N-Ras forms HMW complexes. (A) Cytosol (S350) from MDCK cells (±simvastatin) or rat liver homogenate or bacterially expressed H-Ras were subjected to Superdex 75 size-exclusion chromatography, and endogenous protein levels in each fraction were analyzed by immunoblot. (B) Protein levels of endogenous Ras isoforms in HMW and LMW fractions from A were analyzed by immunoblot. (C) Superdex 200 chromatography of S350 from MDCK cells stably expressing GFP–N-Ras. In A and C, peak elution of various molecular weight standards are indicated at the bottom.