FIGURE 1.

pRP.ExBi-MTIIa-hPSA-eGFP vector EcoRI, NdeI, and XhoI restriction sites correspond to endonucleases with the same names. Recomb LoxP recombination are LoxP sites for Cre-LoxP recombination. F1 ori represents the bacterial origin of replication. The TEV protease cleavage site is a proteolytic cleavage site for TEV protease. Amp R is the β-lactamase gene, which is used for the selection of recombinant bacteria. It corresponds to the SV40-derived eukaryotic origin of replication of simian virus 40. The eGFP reporter corresponds to the fluorescent eGFP reporter gene. The reduced promoter MTIIa (or MTIIa) is followed by the hPSA sequence, which includes the signal peptide, the His-tag and the cleavage site for TEV protease (TEV protease cleavage site) in its initial region. After the target gene, an IRES region and the eGFP reporter gene were included. Recombination Cre/LoxP sites were included flanking promMTIIa-hPSA.