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. 2016 Jun 23;161:2431–2440. doi: 10.1007/s00705-016-2936-2

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 3 — Relative fluorescence lifetime of GFP-MP fusions. Normalised fluorescence lifetime of GFP photon donor (48K-GFP and GFP-2B fusions) (grey bars) and relative fluorescence lifetime of donors in the presence of the PDLP-RFP photon acceptor (black bar). The percentage of decrease in fluorescence lifetime and standard deviations are displayed numerically in each bar. Error bars indicate the standard deviation. An asterisk indicates a significant (P < 0.05) decrease in the donor fluorescence lifetime. N = number of experiments, n = number of fluorescent spots measured