Fig. 4.

Localisation of PDLP and MPs in N. benthamiana protoplasts. a) 48K-GFP expressed from pSOL2095 localises to peripheral spots and short tubules. b) GFP-2B forms protruding tubules when transiently expressed in protoplasts. c) PDLP-GFP localisation to peripheral spots and, to a lesser extent, in internal spots. d) Localisation of internal PDLP-RFP spots with the ER. The latter is visualized using GFP-HDEL. e to g and i to k) Localisation of co-expressed GFP-labelled MPs (e, i) and PDLP-RFP (f, j) (merge in g and k). Arrowheads indicate peripheral PDLP spots. h and l) Extended focus image showing fluorescent 48K and 2B movement tubules (h and l, respectively) and PDLP-RFP at distinct locations, without overlap at the base of the tubule. Chloroplast autofluorescence (a, b) is displayed in magenta. The scale bar in a-g and i-k is 5 µm. The scale bar in h and l is 2 µm