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. 2016 Jul 27;3(4):ENEURO.0029-16.2016. doi: 10.1523/ENEURO.0029-16.2016

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Copyright © 2016 Andrews et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 3. — α9integrin-V5 expressed in adult RGCs is transported into optic nerve axons. Confocal images of flat mount retina show RGCs immunolabeled with anti-V5 (green) and colabeled with anti-β3 tubulin (red) 3 weeks after intravitreal injection of AAV2-CAG-α9integrin-V5 (A, C). A*, A high magnification image (from A) of integrin-containing axons in a fascicle (arrows) travelling toward the optic nerve. Epifluorescent images in B of optic nerve indicate V5-labeled α9integrin within axon fibers of the optic nerve 3 weeks following AAV injection. Arrows in C indicate V5-labeled axons following along the course of β3 tubulin axons. Scale bars: A, C, 20 μm; B, 50 μm.