Double-labeling of Fos with β-gal and NeuN in the OFC and CeA. A, percentage of Fos + β-gal and Fos + NeuN double-labeled neurons in OFC and CeA in adolescent (left) and adult rats (right) perfused after extinction tests on withdrawal day 14. Pictures of double-labeling of adult Fos + β-gal and Fos + NeuN in the OFC (B, C) and CeA (D, E) of adult Fos-lacZ rats that underwent an extinction test on withdrawal day 14. Red, β-gal or NeuN (top); green, Fos (middle); yellow, Fos + β-gal and Fos + NeuN double-labeled neurons (bottom). Scale bar, 50 μm. F, Brain images indicating sampling regions for the OFC (top) and CeA (bottom).