Figure 5.

Daun02 injections into the CeA of adult Fos-lacZ rats decreased incubated nicotine seeking on withdrawal day 14. A, Timeline of the experimental procedure. B, Nicotine self-administration. Data are mean ± SEM of nicotine infusions and active and inactive lever presses of all rats (total n = 42) during the 12 training days. C, Extinction responding. Data are mean ± SEM of presses on the previously active lever and on the inactive lever during the extinction test after 14 withdrawal days. D, Cannula placements. Numbers indicate mm from bregma. E, β-gal expression. Left, Mean ± SEM β-gal-positive neurons in the CeA in the four groups. Right, Representative photomicrographs of β-gal expression in the CeA of rats in the different treatment groups. We injected the rats with Daun02 or vehicle 75 min after a short 15 min extinction session or exposure to a novel context on withdrawal day 11. n = 9–12 per group.