Figure 9.

H&E staining shows mSYMPX reduced paw inflammation in the CFA model. Samples of cross sections of the paw skin taken near the injection site were processed with the H&E method. A, Normal paw skin from animal with no CFA injection. B, Seven days after CFA injection with sham mSYMPX (14 d before CFA injection). C, Seven days after CFA injection with mSYMPX (14 d before CFA injection). There is greater edema, swelling, and immune cell infiltration in B compared with C. Scale bars, 200 μm; low-power inset, 400 μm. Similar results were obtained in 4 male animals per group. D, The paw swelling index (paw width × paw height, normalized to the contralateral paw) was significantly lower in the mSYMPX group on all days tested. Dotted line indicates index of 1 (no swelling). **p < 0.01; ***p < 0.001, significant difference between groups at indicated time points (two-way repeated-measures ANOVA with Bonferroni post hoc test). N = 8 male animals per group (same animals as Fig. 8).