. 2016 Aug 17;36(33):8712–8725. doi: 10.1523/JNEUROSCI.4118-15.2016

Table 2.

Effect of mSYMPX on cytokine profiles after local DRG inflammation^a

	Cytokine	Concentration in normal DRG^b	POD 1 fold change^c LID; LID + mSYMPX	POD 3 fold change LID; LID + mSYMPX	POD 7 fold change LID; LID + mSYMPX	POD 14 fold change LID; LID + mSYMPX
Group 1	IL-2	0.14 ± 0.04	0.29 ± 0.1^*^#	0.14 ± 0.1^**^#	0.19 ± 0.1^**^#	0.26 ± 0.2^*
			1.07 ± 0.4	1.05 ± 0.4	0.71 ± 0.3	1.10 ± 0.7
	IL-12p70	0.85 ± 0.12	0.60 ± 0.1	0.23 ± 0.1^**^#	0.40 ± 0.1^*	0.43 ± 0.1^*
			1.08 ± 0.3	0.67 ± 0.1	0.52 ± 0.1^*	0.68 ± 0.3
	Eotaxin	0.30 ± 0.04	0.59 ± 0.1^*^#	0.40 ± 0.1^**^#	0.38 ± 0.1^**^##	0.47 ± 0.1^*
			1.07 ± 0.1	1.10 ± 0.2	0.81 ± 0.0	0.97 ± 0.2
	IL-4	0.36 ± 0.04	0.97 ± 0.6	0.38 ± 0.2^*^#	0.41 ± 0.2^*	0.29 ± 0.1^**^#
			1.24 ± 0.1	1.05 ± 0.2	0.55 ± 0.1^**	0.84 ± 0.2
Group 2	MCP-1	2.84 ± 0.21	5.1 ± 0.6^***^#	3.6 ± 0.4^***^##	3.0 ± 0.5^***^#	1.9 ± 0.3^*
			2.8 ± 0.5^***	1.9 ± 0.2^**	1.5 ± 0.2	1.1 ± 0.2
	IL-6	1.83 ± 0.37	11.0 ± 3.0^***	3.9 ± 1.1^**^#	1.4 ± 0.3	0.7 ± 0.2
			5.2 ± 0.9^**	1.6 ± 0.1	1.3 ± 0.3	1.0 ± 0.5
	IL-1α	0.53 ± 0.27	10.8 ± 2.6^**^##	21.2 ± 10.7^**^##	10.5 ± 2.6^**^#	3.0 ± 0.9
			2.1 ± 0.5	2.6 ± 0.5^*	2.8 ± 0.8	1.6 ± 0.7
	MIP-2	0.47 ± 0.09	14.7 ± 4.4^***	7.7 ± 3.6^*	1.7 ± 0.2	1.4 ± 0.4
			6.2 ± 2.8^*	1.4 ± 0.2	1.2 ± 0.2	1.2 ± 0.4
	GRO/KC	1.87 ± 0.39	4.9 ± 3.5	3.5 ± 2.6	2.0 ± 0.9	0.7 ± 0.1
			1.3 ± 0.3	1.1 ± 0.3	0.8 ± 0.1	1.0 ± 0.3
Group 3	IL-18	3.53 ± 0.42	2.5 ± 0.3^**	5.6 ± 0.2^***^###	5.8 ± 0.3^***	5.7 ± 1.0^***^#
			1.8 ± 0.3^*	3.2 ± 0.1^***	4.1 ± 0.9^**	2.6 ± 0.8^*
	MIP-1α	0.05 ± 0.01	48.2 ± 13.1^***	37.4 ± 8.8^***^#	33.0 ± 4.9^***	30.0 ± 7.1^***^#
			20.0 ± 8.3^**	11.8 ± 4.0^***	15.5 ± 6.3^**	8.9 ± 7.7
	IL-1β	0.85 ± 0.18	17.2 ± 2.1^***^#	15.3 ± 2.0^***^#	15.1 ± 1.5^***	8.5 ± 1.8^***
			5.9 ± 2.2^**	6.3 ± 1.8^**	8.8 ± 3.2^**	4.5 ± 2.6
Group 4	RANTES	0.14 ± 0.04	1.38 ± 0.2^#	1.39 ± 0.2	2.63 ± 0.8^*	4.37 ± 1.4^*^#
			0.60 ± 0.1	0.93 ± 0.2	3.91 ± 1.3	1.12 ± 0.3
	IFNγ	0.79 ± 0.22	0.90 ± 0.6	4.04 ± 0.6^**	3.34 ± 0.7^*	1.39 ± 0.5
			1.77 ± 0.2	2.49 ± 0.3^*	2.21 ± 0.7	1.26 ± 0.6
	Leptin	2.48 ± 0.67	1.28 ± 0.4	0.95 ± 0.3	1.35 ± 0.2	2.24 ± 0.7
			0.62 ± 0.1	1.27 ± 0.2	2.28 ± 0.4^*	1.73 ± 0.3
	Fractalkine	0.58 ± 0.03	1.22 ± 0.1	1.27 ± 0.2	1.25 ± 0.1	1.41 ± 0.2
			1.34 ± 0.1^**	1.52 ± 0.1^**	1.44 ± 0.1^**	1.29 ± 0.1^*
	VEGF	1.32 ± 0.03	0.79 ± 0.2	1.23 ± 0.2	1.09 ± 0.1	0.72 ± 0.0^**
			0.78 ± 0.1^*	0.95 ± 0.0	0.88 ± 0.1	0.80 ± 0.1
	IP-10	0.72 ± 0.16	5.69 ± 0.9^***	7.14 ± 0.7^***	6.60 ± 0.7^***	4.14 ± 1.0^**
			4.78 ± 1.3^**	6.82 ± 0.9^***	4.16 ± 0.8^**	2.13 ± 0.3^*
	TNFα	2.03 ± 0.44	0.59 ± 0.1	0.51 ± 0.1	0.44 ± 0.1^*^#	0.50 ± 0.1
			0.91 ± 0.2	1.00 ± 0.2	0.70 ± 0.1	0.79 ± 0.2

^aN = 5 normal male rats; 4 male rats per group in all other groups and time points. Both L4 and L5 DRGs were inflamed and combined into a single sample. Absolute values of cytokine concentration shown for normal DRG are in the lysate (see Materials and Methods), depend on method of protein normalization, number of DRG per sample, and sample volume, and hence are not directly comparable with our previous study (Xie et al. 2006).

^bAverage of concentrations in the DRG samples (pg/ml) divided by μg protein.

^cFold change was determined by normalizing values to the average value in normal DRG. Top value in each row, inflamed DRG plus sham mSYMPX; lower value, inflamed DRG plus mSYMPX. mSYMPX or sham mSYMPX was performed 7 d before DRG inflammation.

*p < 0.05;

**p < 0.01;

***p < 0.001; significantly different from normal (ratio t test).

^#p < 0.05;

^##p < 0.01;

^###p < 0.001; significant difference between mSYMPX and sham SYMPX groups (ratio t test).