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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Mol Microbiol. 2015 Sep 25;98(6):1101–1114. doi: 10.1111/mmi.13201

Fig. 2. Inducible knockdown of REX1 using the glmS ribozyme system decreases the number of Maurer’s cleft puncta.

Fig. 2

A. Western blots confirming knockdown of expression of REX1 in REX1_KD transfectants probed with anti-HA and anti-REX1. B,D. Immunofluorescence microscopy of paraformaldehyde-fixed infected RBCs probed with Maurer’s cleft marker anti-MAHRP1 (green) and anti-HA or anti-KAHRP (red). Nuclei are stained with DAPI (blue). Scale bar = 3 μm. C. Quantitation of numbers of Maurer’s clefts produced by REX1_KD transfectants in the presence and absence of 2.5 mM GlcN. The mean number of Maurer’s clefts produced per singly nucleated infected RBC was determined by counting SBP1-labelled puncta in at least 10 cells. Error bars = S.D.