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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Mol Microbiol. 2015 Sep 25;98(6):1101–1114. doi: 10.1111/mmi.13201

Fig. 4. REX1(Δ371–579)-GFP parasites exhibit giant Maurer’s clefts.

Fig. 4

A. REX1(Δ371–579)-GFP-infected RBCs fixed with paraformaldehyde, permeabilized with EqtII and labelled with anti-GFP. B. REX1-GFP and REX1(Δ371–579)-GFP-infected RBCs were fixed with paraformaldehyde/glutaraldehyde and probed with anti-GFP (green) and anti-SBP1 (red). Samples were examined using 3D-SIM. C. REX1(Δ371–579)-GFP-infected RBCs were labelled with BODIPY-ceramide, fixed with paraformaldehyde/glutaraldehyde, labelled with DAPI and imaged using widefield deconvolution microscopy. The right hand panel shows rendering of the surface of the 3D structure using Imaris software. D. REX1(Δ371–579)-GFP-infected RBCs were fixed with paraformaldehyde/glutaraldehyde and probed with anti-EXP2 (green) and anti-REX1 (red). Samples were examined using deconvolution microscopy. Scale bars = 3 μm; zoom bar = 1 μm.