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. 2016 Aug 10;7:12284. doi: 10.1038/ncomms12284

Figure 5. Stability and maintenance of H3K4me3 after DNA demethylation.

Figure 5

(a) Relative EpCAM messenger RNA (mRNA) expression, at each specific time point after using the inhibitor of DNA demethyltransferases 5′aza for 3 days. (b) H3K4me3 ChIP–qPCR enrichment at the promoter region of EpCAM 10 days after demethylation and ZF fusion protein expression. (c) DNA methylation levels at the EpCAM promoter determined by pyrosequencing, black-dot line represents mean methylation levels of untreated cells. (d) H3K79me2 ChIP–qPCR enrichment at the promoter region of EpCAM 3 and 10 days after demethylation and ZF fusion protein expression. (e) H3K79me2 ChIP–qPCR enrichment at the promoter region of PLOD2 3 and 10 days after ZF fusion protein expression. n=3 independent experiments; error bars±s.d.