Skip to main content
. 2016 Aug 17;6:31534. doi: 10.1038/srep31534

Figure 4. Upregulation of p66Shc induced the expression and phosphorylation of p53.

Figure 4

MCF-7 cells were infected with Ad-p66Shc or negative control for 48 h. (A) The cell extracts were prepared and analyzed by Western blot with the corresponding antibodies. The quantification of the digital images was performed using ImageJ software. (B) MCF-7 cells were stained with annexin V-FITC and PI and analyzed by flow cytometry. The data represent the means ± SEM, n = 3 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 vs NC (NC: negative control).