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(a) 293-ErbB2/3 cells were pretreated with 1 mM vanadate for 15 min, and then treated with TPA for 10 min. Whole cell lysates were subjected to immunoblotting with antibodies against pY-ErbB2, ErbB2, pERK, and Actin. (b) Band densities were determined and the level of pY-ErbB2 was calculated as a ratio against total ErbB2 quantity. Values (% of control) represent mean ± S.D. from four independent experiments, *p < 0.01.
