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. 2016 Aug 17;6:31481. doi: 10.1038/srep31481

Figure 2. Phenotypes of CRIPSR/Cas9-induced mutant apples.

Figure 2

(a,b) Clear albino transformants, ex7–20 bp#89A and ex7–20 bp#49A. Arrows indicate albino transformants regenerated around 8 months after transformation. The green shoots are transformants without visible phenotype. (c) Left; wild type ‘JM2’ shoot. Middle; pale green transformant with chimeric mutations, ex7–20 bp#70B. Right; pale green transformant regenerated from the same leaf disk as ex7–20 bp#70B. (d) A typical variegated transformant with chimeric mutations, ex7–20 bp#49C. The most variegated transformant had leaves edged in white to pale green while the central part is green. (e) Pale green transformant targeted on third exon, ex3–20 bp#81B. (f) GFP fluorescence of (e). (g) Albino transformant, ex6-18bp#54. The green shoots are transformants without visible phenotypes. Bars: 1 mm.