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. 2016 Aug 17;6:31595. doi: 10.1038/srep31595

Figure 4. Internalized amount by A549 cells at an Au concentration of 50 μg/mL.

Figure 4

(a) L(D)-MAV-AuNPs and L(D)-PAV-AuNPs in 10% FBS/DMEM, (b) PAV-AuNPs pretreated with 1 mg/mL D-valine or D-PAV (Mw: 18743 Da) in 10% FBS/DMEM, and (c) PAV-AuNPs in serum-free DMEM after co-incubation for 24 h. (d) Influence of pharmacological inhibitors on uptake of L-PAV-AuNPs and D-PAV-AuNPs, respectively. The cells were cultured without or with pretreatment by amantadine-HCl (Aman, 1 mM, inhibitor of clathrin-mediated endocytosis), genistein (Ge, 100 μM, inhibitor of caveolaemediated endocytosis), amiloride-HCl (Amilo, 2 mM, inhibitor of macropinocytosis), cytochalasin D (CytD, 10 μg·mL−1, inhibitor of cytoskeleton), NaN3 (0.1% (w/v), inhibit energy-dependent process) for 1 h, and then cultured with PAV-AuNPs for another 4 h. * and ** indicate significant difference at p < 0.05 and p < 0.01, respectively.