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. 2016 Aug 17;6:31363. doi: 10.1038/srep31363

Figure 3. Anti-BLUE and anti-RAINBOW antibodies can be used over a wide range of exposure times.

Figure 3

A dilution series from 1 μl to 0.25 μl of Bio-Rad Precision Plus Protein All Blue marker (recommended loading volume by the manufacturer is 10 μl per lane) was detected with HRP-coupled anti-BLUE antibody (0.5 mg/ml, diluted 1:5,000; upper panels). A similar dilution series from 1 μl to 0.25 μl of NEB Color Prestained Broad Range protein standard marker (recommended loading volume by the manufacturer is 5 μl per lane) was detected with HRP-coupled anti-RAINBOW antibody (0.4 mg/ml, diluted 1:5,000; lower panels).