Figure 1. Phosphatidic acid modulates the rate of IAPP fibrilization.

IAPP misfolding was measured as a function of time using ThT and demonstrates a dependence upon the mol% of PA. (a) Representative ThT curves from experiments with 12.5 μM human IAPP and 500μM lipid in 10mM phosphate buffer pH 7.4 during incubation with LUVs composed of 66% PA (omitted in A), 25% PA (□), 10% PA (Δ), 1% PA (X), and 0% PA (100% PC, ○). B) Comparison of average t₅₀ values with the mol% PA from experiments in A. Error bars represent one standard deviation from a minimum of 3 experiments per condition. (p < 0.05 for 1%, and p < 0.01 for all other conditions, compared with 100% PC-LUV control).