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. 2016 Aug 17;6:31094. doi: 10.1038/srep31094

Figure 2. IAPP forms an α-helix upon interaction with phosphatidic acid containing LUVs before transitioning into a β-sheet structure.

Figure 2

(a–c) Representative circular dichroism spectra are shown. The secondary structure of 25 μM IAPP was first determined in 10 mM phosphate buffer, pH 7.4 (—). (a) minimum at 202 nm suggests a mostly disordered structure. LUVs composed of 66 (a), 25 (b), and 10 mol% (c) POPA were added and the structure was immediately measured (•••). Minima at 208 nm and 222 nm indicate a transition into an α-helix upon interaction with LUVs. After incubation at room temperature, the samples were measured a final time (— —). A minimum near 218 nm is most consistent with a β-sheet structure. D) Initial spectra of IAPP upon addition of 66 (— • —), 25 (— —), and 10 mol% (•••) PA-LUVs are overlaid with the disordered structure in buffer (—), provided for reference.