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. 2016 Aug 17;6:31094. doi: 10.1038/srep31094

Figure 3. Electron microscopy reveals IAPP fibrils in some cases decorated with PA-LUVs.

Figure 3

12.5 μM IAPP was allowed to misfold in the presence of 500 μM PA-containing LUVs. Samples were incubated for the amount of time required to achieve ThT positivity, as in Fig. 1, before being applied to the micrograph grid. (a–d) EM micrographs of IAPP in 10 mM phosphate buffer, pH 7.4 with 66 (a), 25 (b), 10 (c), and 1 mol% (d) PA-LUVs. These images show fibrils, many of which are decorated with LUVs. In some cases the shape of the LUVs are distorted where they contact fibrils. Lipid free sections of fibrils measure diameters of ~7 nm. Some fibrils assemble laterally, increasing the apparent fibril diameter. Arrows indicate small spherical structures 6 nm in diameter and greater (arrows). Scale bars = 200 nm.