Figure 4. Oleic Acid enhances IAPP misfolding kinetics.

ThT was used to follow IAPP aggregation kinetics. (a) IAPP aggregation was measured as a function of time using ThT and demonstrates dependence upon the mol% of oleic acid. (a,b) Representative ThT curves from experiments with 12.5 μM human IAPP and 500 μM oleic acid in 10 mM phosphate buffer pH 7.4 25% (□), 10% (Δ), 1% oleic acid (x), and 100% POPC (○). (b) Comparison of t₅₀ values with the mol% of oleic acid in LUVs from experiments in (a). Error bars represent one standard deviation from a minimum of 3 experiments per condition. (p < 0.01 for all conditions compared to 100% POPC LUV control).