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. 2016 Aug 17;6:31748. doi: 10.1038/srep31748

Table 1. Assay condition of the receptor binding assay.

  Ligand Kd Bmax Non-specific ligand
MOR Diprenorphine 0.41 nM 3.8 pmol/mg 10 μM Naloxone
KOR Diprenorphine 0.4 nM 1.1 pmol/mg 10 μM Naloxone
DOR Naltrindole 0.49 nM 8.6 pmol/mg 10 μM Naloxone
NOP Nociceptin 1 nM 4.6 pmol/mg 1 μM Orphanin-FQ
Rat brain Naloxone 1.4 nM 0.095 pmol/mg 1 μM Naloxone