Figure 3. Cellular localization of TBN in H23 cells.
Florescence microscopy of H23 cells incubated with TBN (GelFlAb-siRNACy5) and its analogues GelFlAb-siRNA, Gel-AbCy5-siRNA, Gel-Ab-siRNACy5, siRNACy5 and AbCy5. DAPI was used as a nuclear staining marker. Fluorescein was used for encapsulation in Gelatin Nanoparticles. Cy5 was used for labelling antibody and siRNA. The figure shows co-localization of cy5 labelled siRNA and fluorescein encapsulated gelatin nanoparticles in H23 cells. Appropriate controls with and without fluorescein or cy5 were used. All images were recorded at 20X magnification.