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. 2016 Aug 17;3(4):ENEURO.0207-16.2016. doi: 10.1523/ENEURO.0207-16.2016

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Copyright © 2016 Maison et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 4. — Type II terminals are apposed to presynaptic ribbons in Prph^−/− and Prph^+/+ ears. A, B, Maximum projections of confocal z-stacks from the 11.3 kHz region of a knock-out and a wild-type ear, shown in the acquisition plane (x–y) and the orthogonal plane (z–y; Fig. 3C ). Green-filled, red-rimmed arrowheads point to appositions between (parvalbumin-positive) type II terminals and (CtBP2-positive) presynaptic ribbons. Red-filled, white-rimmed arrowheads in the z–y projections point to nonsynaptic ribbons. Scale bar and immunostaining keys apply to all panels. Dashed white boxes in the z–y projections show the z-cropping used to generate the x–y projections.