Table 1. CRISPR-PCS enhances chromosome splitting efficiency.
| Splitting pointa | CRISPR -PCSb | No. of transformants | No. of transformants subjected to karyotype analysis | No. of transformants with expected splitting |
|---|---|---|---|---|
| C15-P1 (CgHIS3) | + | 214 | 10 | 10 |
| − | 1 | 1 | 1 | |
| C16-P1 (CgLEU2) | + | 680 | 10 | 10 |
| − | 3 | 3 | 3 | |
| C15-P1 (CgHIS3)C16-P1 (CgLEU2) | + | 119 | 10 | 5 |
| − | 0 | 0 | 0 |
aOne of the two splitting modules for each splitting site contains a marker gene described in parentheses.
bChromosome splitting was carried out with (+) or without (−) a gRNA expression plasmid in addition to splitting modules.