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. 2016 Aug 17;6:30278. doi: 10.1038/srep30278

Table 1. CRISPR-PCS enhances chromosome splitting efficiency.

Splitting pointa CRISPR -PCSb No. of transformants No. of transformants subjected to karyotype analysis No. of transformants with expected splitting
C15-P1 (CgHIS3) + 214 10 10
1 1 1
C16-P1 (CgLEU2) + 680 10 10
3 3 3
C15-P1 (CgHIS3)C16-P1 (CgLEU2) + 119 10 5
0 0 0

aOne of the two splitting modules for each splitting site contains a marker gene described in parentheses.

bChromosome splitting was carried out with (+) or without (−) a gRNA expression plasmid in addition to splitting modules.