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. Author manuscript; available in PMC: 2016 Aug 17.

Published in final edited form as: Toxicol Sci. 2016 Apr 21;152(1):85–98. doi: 10.1093/toxsci/kfw074

FIG. 2 — T_reg subset expands during dermal TDI sensitization. Flow cytometric analysis of T_regs following dermal TDI sensitization (A) T_regs were first gated on their expression of CD3 and CD4, then were further identified by CD25 and Foxp3 expression at indicated time points. dLN T_reg frequency (B) and number (C) were determined based on flow cytometry analysis and extrapolation of this data with total dLN cellularity. Graph symbols represent mean (± SE) of 5 mice per group. P-values are represented by (0.5% TDI) and asterisks (4% TDI) (P < .05). Significance is indicated as follows: P ≤ .05 (*), P ≤ .01 (**), P ≤ .001 (***), and P ≤ .0001 (****) for 4% TDI or P ≤ .05 (^), P ≤ .01 (), P ≤ .001 (^), and P ≤ .0001 () for 0.5% TDI. Dermal treatment groups are indicated by the following symbols: circle, acetone; square, 0.5% TDI; and triangle, 4% TDI.

Inline graphic — T_reg subset expands during dermal TDI sensitization. Flow cytometric analysis of T_regs following dermal TDI sensitization (A) T_regs were first gated on their expression of CD3 and CD4, then were further identified by CD25 and Foxp3 expression at indicated time points. dLN T_reg frequency (B) and number (C) were determined based on flow cytometry analysis and extrapolation of this data with total dLN cellularity. Graph symbols represent mean (± SE) of 5 mice per group. P-values are represented by (0.5% TDI) and asterisks (4% TDI) (P < .05). Significance is indicated as follows: P ≤ .05 (*), P ≤ .01 (**), P ≤ .001 (***), and P ≤ .0001 (****) for 4% TDI or P ≤ .05 (^), P ≤ .01 (), P ≤ .001 (^), and P ≤ .0001 () for 0.5% TDI. Dermal treatment groups are indicated by the following symbols: circle, acetone; square, 0.5% TDI; and triangle, 4% TDI.