FIG. 6.
The severity of the sensitization response is intensified in the absence of Tregs during TDI sensitization. Tregs were depleted in mice prior to and during dermal TDI sensitization (A). Evaluation of dermal irritancy (B), dLN cellularity (C), dLN Th2 population (D), dLN IL-4 gene expression (E), and serum total IgE (F) were evaluated following a single exposure of 0.5 or 2% TDI with isotype control or anti-CD25. Bars represent mean (± SE) of 5 mice per group. Significance is indicated as follows: P ≤ .05 (*), P ≤ .01 (**), P ≤ .001 (***), and P ≤ .0001 (****). P values are represented by asterisks (comparison of acetone to TDI-exposed group from the same antibody treatment regimen) or horizontal bars with asterisks above (comparison of antibody and isotype-treated groups receiving identical chemical treatment).