TABLE 2.

dLN Migratory Effector T_reg Population Expands During TDI Sensitization

CCR6+ % (of Tregs)	1 day	2 day	4 day	7 day	9 day
Acetone	11.14 ± 0.7	12.72 ± 1.03	10.86 ± 0.55	18.22 ± 1.36	9.23 ± 0.8
0.5% TDI	10.22 ± 0.68	17.7 ± 1.64**	20.58 ± 0.84****	26.54 ± 0.68****	14.82 ± 0.99**
4% TDI	10.02 ± 0.92	10.2 ± 1.1	30.7 ± 1.75****	40.42 ± 1.87****	25.02 ± 1.98****
CCR6+ #(× 105 cells)
Acetone	0.2 ± 0.05	0.14 ± 0.04	0.19 ± 0.02	0.24 ± 0.04	0.16 ± 0.03
0.5% TDI	0.34 ± 0.06*	0.61 ± 0.12****	2.41 ± 0.19****	2.35 ± 0.28****	0.57 ± 0.13****
4% TDI	0.533 ± 0.05****	0.3 ± 0.07**	5.25 ± 0.48****	5.59 ± 0.41****	1.99 ± 0.2****
CD103+ % (of Tregs)	1 day	2 day	4 day	7 day	9 day
Acetone	22.86 ± 0.75	18.88 ± 4.24	20.82 ± 0.94	28.44 ± 2	21.18 ± 0.92
0.5% TDI	18.38 ± 0.84	24.44 ± 2.99*	35.88 ± 0.84****	38.8 ± 1.1***	32.72 ± 2.23****
4% TDI	19.62 ± 1.25	16.5 ± 1.64	43.18 ± 1.78****	52.12 ± 1.36****	44.64 ± 1.45****
CD103+ #(× 105 cells)
Acetone	0.41 ± 0.09	0.16 ± 0.03	0.37 ± 0.04	0.38 ± 0.07	0.36 ± 0.06
0.5% TDI	0.59 ± 0.1	0.83 ± 0.15****	4.21 ± 0.33****	3.47 ± 0.45****	1.22 ± 0.23****
4% TDI	1.04 ± 0.06****	0.48 ± 0.11****	7.4 ± 0.63****	7.23 ± 0.56****	3.53 ± 0.23****
ICOS+ % (of Tregs)	1 day	2 day	4 day	7 day	9 day
Acetone	13.38 ± 0.36	15.92 ± 1.21	13.56 ± 0.41	18.7 ± 1.22	15.98 ± 1.24
0.5% TDI	14.06 ± 1.03	36.42 ± 2.16****	47.8 ± 1.15****	38.38 ± 1.72****	33.36 ± 2****
4% TDI	16.68 ± 1.45	33.18 ± 1.27****	62.64 ± 1.03****	55.02 ± 2.12****	45.54 ± 1.78****
ICOS+ # (× 105 cells)
Acetone	0.23 ± 0.05	0.17 ± 0.04	0.24 ± 0.02	0.25 ± 0.04	0.28 ± 0.5
0.5% TDI	0.46 ± 0.09**	1.31 ± 0.27****	5.61 ± 0.45****	3.42 ± 0.45****	1.27 ± 0.26****
4% TDI	0.88 ± 0.09****	0.94 ± 0.18****	10.68 ± 0.62****	7.62 ± 0.62****	3.62 ± 0.29****

Data represents mean frequency and number of each T_reg population 1–9 days following chemical exposure. Significance is indicated as follows:

^*P ≤ .05

^**P ≤ .01

^***P ≤ .001

^****and P ≤ .0001 for each group compared with the acetone control value from the matching time point.