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. 2016 Aug 17;6:31646. doi: 10.1038/srep31646

Figure 2. Aβ42 increases Reelin expression in cell culture and is trapped into Aβ fibers.

Figure 2

(a) Differentiated SH-SY5Y cells were treated with 10 μM of Aβ42 and mRNA Reelin was determined and compared with non-treated (NT) cells. Data represents relative Reelin mRNA levels normalized to GAPDH and are expressed as mean values ± SEM of 10 independent determinations from at least 2 different experiments (*p < 0.001). (b) Western blots of SH-SY5Y cell extracts, the media and the pellet from the media of cells maintained in the presence or absence of Aβ42, and probed for Reelin (representative blot, n = 3) and α-tubulin from cell extracts as a loading control. (c) Proteins recovered in the pellet from the media were also probed with the 4G8 antibody against Aβ.