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. 2016 Aug 17;6:31646. doi: 10.1038/srep31646

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Copyright © 2016, The Author(s)

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AD subjects were categorized as early (I-II to IV) or advanced Braak stages (V to VI). (a) Relative Reelin mRNA expression analyzed by qRT-PCR in frontal cortex samples from ND (n = 11) and AD subjects (stages I to IV, n = 7; stages V to VI, n = 10). (b) Reelin mRNA was also analyzed in the hippocampus of ND controls (ND; n = 5) and AD samples corresponding to Braak stages IV to VI (n = 9). The values were calculated from relative standard curves and normalized to GAPDH from the same cDNA preparation, confirming the specificity of the PCR products from dissociation curves. The data represent the means ± SEM. *p < 0.05 using Student’s t-test.