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. 2016 Aug 17;6:31644. doi: 10.1038/srep31644

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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A cas locus located between two CRISPR arrays (CRISPR1 and CRISPR2) in K. pneumoniae NTUH-K2044 strain was revealed. CRISPR1 array composed of four repeated sequences (solid diamond) and three spacer sequences (open square) was located at nucleotides 3011356 to 3011567. CRISPR2 array composed of twenty-three repeated sequences and twenty-two spacer sequences was located at nucleotides 3001234 to 3002603. Sequences of spacer 2 flanked by repeat sequences in CRISPR2 and engineered proto-spacer 2 (sequences matching spacer 2 and putative PAM) are shown.