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. 2016 Aug 17;6:31644. doi: 10.1038/srep31644

Figure 2. The transformation efficiencies of pUC-4K and pUC-4K with engineered proto-spacer 2 identical with spacer 2 of CRISPR2 in NTUH-K2044 wild type, hns deletion (∆hns), hns and cas3 double deletion (∆hns∆cas3) and hns complementation strains.

Figure 2

The transformation efficiency of pUC-4K was set as 100% and that of proto-spacer2(CTT)::pUC-4K was calculated accordingly. Data are presented as means ± SEM from three independent experiments.