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. 2016 Aug 17;6:31644. doi: 10.1038/srep31644

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The transformation efficiency of pUC-4K was set as 100% and those of CRISPR1-spacer2(TTT)::pUC-4K and CRISPR1-spacer2′(TTT)::pUC-4K were calculated accordingly. The transformation of proto-spacer2(TTT) served as a positive control. Data are presented as means ± SEM from three independent experiments.