Table 4. Primers used for PCR, RT-PCR and real-time PCR.

Primer	Sequence	Probe (Roche)
Genotyping
a	5′-CTTCCTGCCTCCTCTTCATCATCAGGTATTG-3′ (NT165773.2)a
b	5′-GTTCAGACAGTGTCAGACCACCTGGAGAAGC-3′ (NT165773.2)a
c	5′-CCTCTGACCTCCCAATGTGTGCATGACAC-3′ (NT165773.2)a
d	5′-GTCCAGCCGAACCTTCTTCAGAGCTACTAG-3′ (NT165773.2)a
Transcription detection
Cdk12	(F) 5′-CAGCTACTACCCCTCCACCTCAGACAC-3′ (NM_026952.2)a
	(R) 5′-GTCCAGCCGAACCTTCTTCAGAGCTACTAG-3′ (NM_026952.2)a
Real-time PCR
Atr	(F) 5′-CCCATGCGAATCATGACC-3′	104
	(R) 5′-ACATCATCGAAGCCTGCAA-3′
Brca1	(F) 5′-ACGAGTCAGGGATGGGAAG-3′	46
	(R) 5′-CCTGAGAGGGGGAGTTTCTC-3′
Cyclin K	(F) 5′-TCCCAAACTTGAGGCTACTCAC-3′	67
	(R) 5′-GAGGCTTCCGGTCTGGAG-3′
Fancd2	(F) 5′-AACGAAGGGAAGCAACTGG-3′	29
	(R) 5′-CTAGCCGACGTCTTTTGGAA-3′
Fanci	(F) 5′-CACCCTAACAGCATTCGTCA-3′	6
	(R) 5′-CGACAGCTTCACCAGTTTTTC-3′
Tbp	(F) 5′-GGCGGTTTGGCTAGGTTT-3′	104
	(R) 5′-GGGTTATCTTCACACACCATGA-3′

^aAccession number of the reference sequence