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. 2016 Aug 15;11(8):e0160092. doi: 10.1371/journal.pone.0160092

Fig 6. Diameter of COPD and healthy subject myotubes after ascorbic acid treatment.

Fig 6

Representative images of COPD myotubes (A) or healthy subject myotubes (C) with or without ascorbic acid treatment, showing fluorescence double-labeling using an anti-troponin T antibody (green) and Hoechst (blue). Bar = 200 μm. Analysis of the variation of the COPD myotube diameter (B) or the healthy subject myotube diameter (D) after ascorbic acid treatment. (E) Diameter of myotubes from healthy subjects and COPD patients, cultured with or without ascorbic acid. (*) and (***) indicate statistical significance at P≤0.05 and P<0.001, respectively. (n.s.) indicates statistically non-significant. The medians are indicated.