Fig 9. ROS production and COPD myotube diameter after both H₂O₂ and ascorbic acid treatment.

(A) The variation of ROS production in myotubes derived from 4 COPD patients is shown after H₂O₂ treatment, and both H₂O₂ and ascorbic acid treatment. (B) Representative images of COPD myotubes from one patient, in absence of treatment, after H₂O₂ treatment or after both H₂O₂ and ascorbic acid treatment, showing fluorescence double-labeling using an anti-troponin T antibody (green) and Hoechst (blue). Bar = 200 μm. (C) Analysis of the variation of the myotube diameter to a reference value of 100after H₂O₂, and both H₂O₂ and ascorbic acid treatments. (n.s.) indicates statistically non-significant. (*) indicates statistical significance at P≤0.05. The medians are indicated.