Fig. 2.

Substrate main chain conformation is likely to be conserved throughout PP5 and PPP family members. Surface representation of (A and B) PP5 and (C and D) Cdc37 substrate binding residues colored according to sequence conservation using ConSurf (Methods). A and C show conservation between PP5 homologs; B and D show conservation among PPP family phosphatases. Cdc37 substrate is shown as sticks, colored as in Fig. 1A, and labeled from −4 to +2 to identify location relative to phosphomimetic S13E (position 0).