Table S2.

Residue variety (%) by amino acid position generated using ConSurf for residues lining the substrate binding cleft (Methods)

PP5 amino acid	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Maximum, % identity/similarity
PP5 family*
R275														100						R 100.000
N308			7.246						1.449		62.319				4.348	18.841	4.348		1.449	N 62.319
M309										100										M 100.000
I312								49.275	1.449	46.377							2.899			I/M/V 98.511
Y313																			100	Y 100.000
P375									1.449			98.551								P 98.551
P376			1.449									98.551								P 98.551
W386						1.449												98.551		W 98.551
R400														100						R 100.000
E428				100																E 100.000
V429		1.471							1.471								97.059			V 97.059
Y451																			100	Y 100.000
PPP superfamily†
R275														100						R 100.000
N308							20.69				5.747		39.08		31.034	1.149	1.149		1.149	Q 39.080
M309		1.149						67.816	20.69	10.345										I/L/M 98.851
I312								34.483		5.747							39.08		20.69	I/M/V 79.31
Y313					20.69														79.31	Y 79.310
P375								14.943				29.885					55.172			I/V 70.115
P376												100								P 100.000
W386																		100		W 100.000
R400														100						R 100.000
E428				32.184									67.816							Q/E 100.00
V429	20.69					1.149			40.23								37.931			L/V 78.161
Y451																			100	Y 100.000

Numbering is the same as for PP5 catalytic domain.

^*In total, 69 homologs were identified using BLAST with identity greater than 56% throughout both the TPR and catalytic domains.

^†In total, 87 homologs were identified using UniProt (>10 homologs for each of the PPP family members). The exception is PP7, for which only Arabidopsis was included.