Table S1.
Cryo-EM data statistics
| RV-C15a full particle | RV-C15a empty particle | |
| EM data collection and processing | ||
| Microscope | Titan Krios | Titan Krios |
| Accelerating voltage, kV | 300 | 300 |
| Camera | Gatan K2 Summit | Gatan K2 Summit |
| Pixel size, Å/pixel* | 1.04 | 1.04 |
| Dose rate, e−/(pixel⋅s) | 8 | 8 |
| Total dose, e−/Å2 | 25.7 | 25.7 |
| No. of particles for the final reconstruction | 8,973 | 3,614 |
| Resolution, ņ | 2.79 | 3.16 |
| Map sharpening B factor, Å2 | −108.6 | −122.2 |
| Model statistics | ||
| Correlation coefficient (around atoms)‡ | 0.885 | 0.880 |
| No. of atoms | ||
| Protein | 6,221 | 6,007 |
| Water | 60 | 0 |
| Average B factor, Å2 | 24.1 | 31.7 |
| rmsd§ | ||
| Bond lengths, Å | 0.010 | 0.010 |
| Bond angles, ° | 0.960 | 0.944 |
| Ramachandran plot§ | ||
| Favored, % | 93.4 | 92.0 |
| Allowed, % | 6.5 | 7.6 |
| Outliers, % | 0.1 | 0.4 |
*
Pixel size in superresolution mode. The physical pixel size is 2.08 Å per pixel.
†
Resolution was determined by the Fourier shell correlation between two half-maps using 0.143 as a cutoff value.
‡
Real-space correlation coefficient (around atoms) between the final EM map and a density map calculated based on the coordinates.
§
Based on the criteria of MolProbity.