Table 1.
Developmental ability of oocytes differentiated from PGCs after growth, maturation, and fertilization in vitro
| Condition for organ culture | No. of cultured gonads | No. of cultured follicles | No. of survived follicles | No. of collected COCs | No. of oocytes matured into MII | No. of eggs fertilized normally | No of embryos developed to two-cell | No. of embryos developed to pup | No. of pups from a gonad |
| FBS/SPS | 9 | 216 | 96 (44%) | 60 (63%) | 46 (77%) | 18 (39%) | 15 (83%) | 6 (40%) | 0.7 |
| FBS/1 µM ICI | 16 | 641 | 470 (73%) | 340 (72%) | 321 (94%) | 168 (52%) | 155 (92%) | 31 (20%) | 1.6 |
| FBS/5 µM ICI | 6 | 312 | 223 (71%) | 178 (80%) | 168 (94%) | 94 (56%) | 91 (97%) | 20 (22%) | 3.3 |
| FBS/10 µM ICI | 6 | 505 | 337 (67%) | 281 (83%) | 256 (91%) | 148 (58%) | 138 (93%) | 19 (14%) | 3.2 |
| Vitrification | 4 | 87 | 57 (66%) | 39 (68%) | 37 (95%) | 19 (51%) | 15 (79%) | 2 (13%) | 0.5 |
| GV (control) | 19 | 155 | 139 (90%) | 70 (45%) | 67 (96%) | 40 (60%) | 2.1 |