Fig. 2.

Combined GC B-cell–specific deletion of relb and nfkb2 impairs the GC reaction. (A) relb^fl/flCγ1-Cre, nfkb2^fl/flCγ1-Cre, or relb^fl/flnfkb2^fl/flCγ1-Cre mice and the corresponding heterozygous and Cγ1-Cre control mice were analyzed by flow cytometry 14 d following immunization with SRBCs for CD95^hiPNA^hi splenic GC B cells. Summary of the frequencies of GC B cells (Lower). Each symbol represents a mouse. Statistical significance was determined by Student’s t test (*P < 0.05; **P < 0.01). Data are shown as mean ± SD. (B) Spleen sections from the indicated genotypes were analyzed for the expression of BCL6 and IgM via IHC.