Fig. 4.

Deletion of nfkb2 in GC B cells impairs the development of antigen-specific PCs. (A) relb^fl/flCγ1-Cre and (B) nfkb2^fl/flCγ1-Cre and the corresponding heterozygous and Cγ1-Cre control mice were analyzed for NP₉-specific IgG1 levels via ELISA (Upper Left) and NP₂₅-specific IgG1 ASCs via ELISPOT (Lower) 28 d following immunization with NP-KLH. Summary of the frequencies of NP₂₅-specific IgG1 ASCs (Right). Each symbol represents a mouse. Statistical significance was determined by Student’s t test (*P < 0.05; ***P < 0.001). Data are shown as mean ± SD.